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Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia. |
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| Authors: | F S Vilim V Alexeeva L L Moroz L Li T P Moroz J V Sweedler and K R Weiss |
| Institution: | a Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA b Whitney Laboratory, University of Florida, St. Augustine, FL 32086, USA c Department of Chemistry & Beckman Institute, University of Illinois, Urbana, IL 61801, USA |
| Abstract: | The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides. |
| Keywords: | Cerebral peptide 2 Neuropeptide Aplysia californica cDNA cloning Peptide processing In situ hybridization Single cell RT-PCR MALDI-TOF MS |
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