Indoleacetic Acid Oxidases in Resting Cereal Grains |
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Authors: | SEPPO LAUREMA |
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Institution: | Department of Biochemistry, University of Helsinki, Unioninkatu 35, SF-00170 Helsinki 17, Finland |
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Abstract: | Peroxidases oxidizing indoleacetic acid (IAA) are present in the resting grains of barley, rye and wheat. The grains also contain small molecular inhibitors of the enzymes. A partly purified preparation of barley seed proteins was shown to contain at least two IAA oxidases about pI 5 and pI 10, mol. weight 30,000 and with a pH optimum 5.1–5.7. The enzymes require Mn2+, 2,4-dichlorophenol and orthophosphate for the maximum activity. Attempts to separate IAA oxidase and peroxidase activities of the enzymes were unsuccessful. |
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