In vitro effects of cryoprotective agents on the response of murine T and B lymphoid subpopulations to mitogenic agents |
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| Authors: | D M Strong A A Ahmed K W Sell D Greiff |
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| Affiliation: | 1. Naval Medical Research Institute, Bethesda, Maryland 20014 U.S.A.;2. Medical College of Wisconsin, Milwaukee, Wisconsin 53233 U.S.A.;1. Job Results Management Institute, 2544 Wakewood Hill Court Winston-Salem, NC 27106, U.S.A.;2. Sheldon B. Lubar School of Business, University of Wisconsin, 3202 N. Maryland Avenue Milwaukee, WI 53202, U.S.A.;1. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China;2. The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China;1. Johns Hopkins University School of Medicine, Baltimore, MD;2. New York University School of Medicine, New York, NY;3. Memorial Sloan-Kettering Cancer Center, Weill Cornell Medical College, New York, NY;4. University of Colorado School of Medicine, Denver, CO;5. Veterans Affairs Eastern Colorado Health Care System, Denver, CO;6. Indiana University School of Medicine, Indianapolis, IN;7. University of Nebraska Medical Center, Omaha, NE;8. Wayne State University School of Medicine, Detroit, MI;9. Cooper Medical School of Rowan University, Camden, NJ |
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| Abstract: | Based on the ability of murine thymic derived lymphocytes (T-cells) to respond to phytohemagglutinin (PHA) and concanavalin-A (Con-A) and bursa equivalent or bone marrow derived lymphoid cells (B-cells) to respond to endotoxin (LPS) and pokeweed mitogen (PWM), a culture system was developed to study the effects of cryoprotective additives on the response of murine subpopulations to the various mitogens. It was found that glycerol and polyvinylpyrolidone (PVP) at various concentrations inhibited the response of spleen cells to all the mitogens. Dimethylsulfoxide (DMSO) at concentrations of 0.25–1.5% (0.03–0.19 m) significantly augmented the uptake of 3H-TdR in spleen cells stimulated with PHA-M or Con-A. Similar concentrations of DMSO depressed the response to LPS and PWM. Possible mechanisms are discussed. |
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