Growth of Bifidobacterium bifidum in whey-based media |
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Authors: | R Mahalakshmi V V P S Murthy |
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Affiliation: | (1) School of Biotechnology, Institute of Post-Graduate Studies and Research, Jawaharlal Nehru Technological University, Hyderabad 500 028, India, IN |
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Abstract: | Bifidobacteria play an important role in human health including the enhancement of resistance against infection in infants. To develop an inexpensive whey-based medium for Bifidobaterium bifidum, potential growth promoters — yeast extract, casein, bovine casein digest, tryptone, peptone and glucosamine — singly or in combinations, were evaluated for their bifidus growth-promoting activity. The effect of environmental conditions on growth in cheese whey was also evaluated. A whey-based medium for B. bifidum was formulated. Cheese whey supplemented with N-acetylglucosamine (1 mg/ml) and yeast extract (10 mg/ml) in the presence of sodium thioglycolate (0.1%) at pH 6.8 promoted the growth of B. bifidum at 37°C. Journal of Industrial Microbiology & Biotechnology (2000) 25, 177–179. Received 20 May 2000/ Accepted in revised form 20 July 2000 |
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Keywords: | : B. bifidum cheese whey growth promoters medium development |
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