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Rapid Construction of a Random Genomic Library from Date-Palm (Phoenix Dactylifera L.)
Authors:Hela  Sakka  Ali  Ould Mohamed Salem  Mokhtar   Trifi  Abdelmajid   Rhouma  Mohamed   Marrakchi
Affiliation:(1) Laboratoire de Génétique et Biologie Moléculaire, Faculté des Sciences de Tunis, 1060 Le Belvédère Tunis, Tunisie;(2) Centre de Recherches Phoenicicoles, INRA, Degache, Tunisie
Abstract:A random genomic library of Tunisian date-palm varieties has been built from total cellular DNA, previously amplified according to an RAPD procedure. The resultant recombinant DNA is characterised by a size ranging from 200 to 1600 bp inserts. This DNA would constitute a large number of anonymous probes useful in Southern hybridisation experiments. It would also provide potential markers aimed at the molecular characterisation of date-palm varieties, aid the search of those associated with bayoud disease and suggest a sex determination of trees.
Keywords:Date-palm  DNA library  RAPD
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