Development of DNA electrochemical biosensor based on immobilization of ssDNA on the surface of nickel oxide nanoparticles modified glassy carbon electrode |
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| Authors: | Noorbakhsh Abdollah Salimi Abdollah |
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| Institution: | aDepartment of Chemistry, University of Kurdistan, P.O. Box 416, Sanandaj, Iran;bResearch Center for Nanotechnology, University of Kurdistan, P.O. Box 426, Sanandaj, Iran;cDepartment of Nanotechnology Engineering, Faculty of Advanced Science and Technology, University of Isfahan, 81746-73441 Isfahan, Iran |
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| Abstract: | A sensitive electrochemical method for DNA hybridization based on immobilization of DNA probe and Ru(NH3)5Cl]PF6 complex onto nickel oxide nanomaterials (NiOxnp) modified glassy carbon electrode was developed. Due to strong affinity of NiOxnp for phosphate groups, oligonucleotides probe with a terminal 5′-phosphate group was attached to the surface of the modified electrode. DNA immobilization and hybridization were characterized by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry using K3Fe(CN)6/K4Fe(CN)6 and Ru(NH3)5Cl]PF6 as probe and indicator, respectively. The Ru-complex current response indicates only the complementary sequence showing an obvious current signal in comparison to non-complementary and three or single point mismatched sequences. The fabricated biosensor possessed good selectivity and sensitivity for complementary probe, taxon: 32630 tumor necrosis factor (TNF). The linear dynamic range, sensitivity and detection limit of the proposed biosensor were 4 × 10−10 M to 1 × 10−8 M, 34.32 nA nM−1 and 6.8 × 10−11 M, respectively. Excellent reproducibility and stability, quite simple and inexpensive preparation are the other advantages of proposed biosensor. |
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| Keywords: | Nickel oxide nanoparticles DNA hybridization Electrochemical sensor Taxon: 32630 tumor necrosis factor (TNF) |
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