BAC library for the amphipod crustacean,Parhyale hawaiensis |
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| Authors: | Ronald J Parchem Francis Poulin Andrew B Stuart Chris T Amemiya Nipam H Patel |
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| Institution: | 1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA;2. Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA;3. Center for Integrative Genomics, University of California, Berkeley, CA 94720-3200, USA;4. Genome Resource Center, Benaroya Research Institute, 1201 Ninth Avenue, Seattle, WA 98101, USA |
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| Abstract: | Bacterial artificial chromosomes (BACs) are capable of propagating large fragments of DNA and have become an invaluable tool for studying genome biology. To fill a phylogenetic gap in available genomic sequence and to complement ongoing molecular and genetic studies, we have generated a BAC library for the marine amphipod crustacean, Parhyale hawaiensis. The library was generated from genomic DNA isolated from whole adult animals and comprises 129,024 individual clones. The median insert size is ～ 140 kb and the genomic coverage is approximately five genome equivalents. We have screened the Parhyale BAC library for developmentally relevant genes and characterized the genomic organization of four genes: a hedgehog ortholog, and three Pax3/7 paralogs. Preliminary analysis suggests that introns are larger and more prevalent in Parhyale than in other arthropods whose genomes have been sequenced, which may partly account for the large genome size in Parhyale. |
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