Expression of hSef in various human tissues and cell lines |
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| Authors: | Huang Guanrong Xiong Shiqin Zhao Qiuhui Wang Yinyin Reng Fangli Ye Xiongjun Chang Zhijie |
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| Affiliation: | (1) Tsinghua Institute of Genome Research, Department of Biological Sciences and Biotechnology, Institute of Biomedicine, Tsinghua University, Beijing, 100084, China;(2) The Third Hospital, Beijing University, Beijing, 100083, China |
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| Abstract: | Sef is a transmembrane protein inhibiting FGF signaling. To determine the correlation of Sef with human diseases, Sef expression patterns were observed in cell lines and human cancer tissues. Western blot using anti-hSef antibodies showed that hSef, when expressed in Cos7 cells gave a molecular mass of 100 KD as compared with 80 KD in an in vitro translation assay suggesting occurrence of glycosylation at the potential N-linked glycosylation sites in the extracellular domain. Northern blot showed that hSef was mainly expressed in human kidney and testis. RT-PCR analysis showed a widely spread expression pattern in several cell lines. Immunohistochemical analysis revealed a high expression level of hSef in kidney, testis, and the corresponding carcinoma tissues. Results demonstrated that Sef might be up-regulated in the cancer tissues suggesting a possible role of Sef in pathophysiology of human diseases. __________ Translated from Chinese Journal of Biochemistry and Molecular Biology, 2005, 21 (2) [译自: 中国生物化学与分子生物学报, 2005,21(2)] |
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| Keywords: | Sef FGF expression pattern human tissue cell line |
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