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Sequences affecting the regulation of solvent production in<Emphasis Type="Italic"> Clostridium acetobutylicum</Emphasis>
Authors:Miles?C?Scotcher  Ke-xue?Huang  Mary?L?Harrison  Frederick?B?Rudolph  Email author" target="_blank">George?N?BennettEmail author
Institution:(1) Department of Biochemistry and Cell Biology, MS 140, Rice University, 6100 Main Street, Houston, TX 77005, USA;(2) Present address: Dow Agrosciences LLC, R & D Discovery, 9330 Zionsville Road, Indianapolis, IN 46268, USA
Abstract:The high solvent phenotype of Clostridium acetobutylicum mutants B and H was complemented by the introduction of a plasmid that contains either an intact or partially-deleted copy of solR, restoring acetone and butanol production to wild-type levels. This demonstrates that the solR open reading frame on pSOLThi is not required to restore solvent levels. The promoter region upstream of alcohol dehydrogense E (adhE) was examined in efforts to identify sites that play major roles in the control of expression. A series of adhE promoter fragments was constructed and the expression of each in acid- and solvent-phases of growth was analyzed using a chloramphenicol acetyl-transferase reporter system. Our results show that a region beyond the 0A box is needed for full induction of the promoter. Additionally, we show that the presence of sequences around a possible processing site designated S2 may have a negative role in the regulation of adhE expression.
Keywords:Clostridium  Solvent            Sol operon  CAT reporter  Alcohol dehydrogenase E
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