X-ray enabled detection and eradication of circulating tumor cells with nanoparticles |
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Authors: | Mainul Hossain Yang Luo Zhaoyong Sun Chaoming Wang Minghui Zhang Hanyu Fu Yong Qiao Ming Su |
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Affiliation: | NanoScience Technology Center, University of Central Florida, Orlando, FL 32826 USA; School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32826 USA. |
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Abstract: | The early detection and eradication of circulating tumor cells (CTCs) play an important role in cancer metastasis management. This paper describes a new nanoparticle-enabled technique for integrated enrichment, detection and killing of CTCs by using magnetic nanoparticles and bismuth nanoparticles, X-ray fluorescence spectrometry, and X-ray radiation. The nanoparticles are modified with tumor targeting agents and conjugated with tumor cells through folate receptors over-expressed on cancer cells. A permanent micro-magnet is used to collect CTCs suspended inside a flowing medium that contains phosphate buffered saline (PBS) or whole blood. The characteristic X-ray emissions from collected bismuth nanoparticles, upon excitation with collimated X-rays, are used to detect CTCs. Results show that the method is capable of selectively detecting CTCs at concentrations ranging from 100-100,000cells/mL in the buffer solution, with a detection limit of ~100CTCs/mL. Moreover, the dose of primary X-rays can be enhanced to kill the localized CTCs by radiation induced DNA damage, with minimal invasiveness, thus making in vivo personalized CTC management possible. |
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