Drug distribution studies with microdialysis. II. Caffeine and theophylline in blood, brain and other tissues in rats |
| |
Authors: | L St?hle S Segersv?rd U Ungerstedt |
| |
Institution: | Department of Pharmacology, Karolinska Institute, Stockholm, Sweden. |
| |
Abstract: | Microdialysis was applied to estimate the pharmacologically active concentration of caffeine and theophylline in blood, adipose tissue, muscle, liver and brain of rats. The concentration of the drugs in the extracellular space was estimated by perfusion with varying concentrations of the drug through the microdialysis probe (difference method). Caffeine (20 mg/kg) was found to be evenly distributed with a free concentration of approximately 120 microM. Theophylline concentration in the brain was 91 microM and in other tissues approximately 120 microM. The rate of penetration into brain extracellular space was higher for caffeine than for theophylline. It is suggested that the lower levels of theophylline attained in the brain may to some extent explain the differences in clinical action profile between caffeine and theophylline. |
| |
Keywords: | |
|
|