Species-specific probe, based on 18S rDNA sequence, could be used for identification of the mucilage producer microalga Gonyaulax fragilis (Dinophyta) |
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Authors: | M Riccardi F Guerrini F Tinti L Boni and R Pistocchi |
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Institution: | (1) Centro Interdipartimentale di Ricerche sulle Scienze Ambientali (CIRSA), Alma Mater Studiorum University of Bologna , Via S. Alberto 163, 48100 Ravenna, Italy |
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Abstract: | In the Adriatic Sea, the correlation between mucilage phenomena and the presence of Gonyaulax fragilis (Schütt) Kofoid (Dinophyta) has been recently demonstrated. The application of PCR-based methods and the development of species-specific
molecular probes might represent powerful technologies for rapid and specific monitoring of microalgal species in seawater
samples. Here, we report sequencing of the small subunit (SSU) ribosomal RNA gene (18S rDNA) of G. fragilis and its comparative analysis within the Dinophyta. Total DNAs were extracted and amplified from cultured cells of G. fragilis, which were isolated from natural phytoplanktonic association in the northern Adriatic Sea. Total 18S rDNA gene was amplified
using 16S1N and 16S2N primers and sequenced using ad hoc designed internal primers. The primers amplified a product of expected size (length 1700/1800 bp). The phylogenetic analysis
carried out by comparing G. fragilis sequence to homologous sequences of Lingulodinium polyedrum (Stein) Dodge, Gonyaulax spinifera (Claparède et Lachmann) Diesing, Protoceratium reticulatum (Claparède et Lachmann) Bütschli revealed a great nucleotide divergence of G. fragilis SSU sequence. Therefore, the SSU sequence could be used as species-specific marker for the identification of this mucilage
producer microalga. In addition, such sequence could be used as target to design oligonucleotide probes for the construction
of DNA microchips as diagnostic tool for the routine monitoring of harmful algae in seawater.
An erratum to this article is available at . |
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Keywords: | Gonyaulax fragilis 18S rDNA Mucilage Adriatic Sea |
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