Potato transformation by T-DNA Cytokinin synthesis gene |
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| Authors: | M Ondrej Ivana MachÁĉková J ĈatskÝ J Eder M Hrouda Jana Pospisilová Helena Synkova |
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| Institution: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Vokovice, Ke dvoru 15/16, CS-160 00 Praha 6;(2) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1;(3) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na sádkách 702, CS-370 05 Ĉeské Budějovice, Czechoslovakia |
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| Abstract: | Potato plants were transformed by the A.tumefaciens vector which integrates into the plant genome two foreign genes only: pTi C58 T-DNA gene 4 (ipt) responsible for elevated
synthesis of cytokinins and kanamycin resistance gene. Three teratoma clones studied showed approximately 3 times, 6 times
and 9 times increased levels of zeatin riboside and zeatin in comparison with untransformed controls and slight increase of
the IAA level. Shoots formed roots in agar cultures sporadically, if the increase of zeatin riboside and zeatin levels was
not higher than 6 times the control level. The chlorophyll content and net photosynthetic rate decreased with increasing levels
of zeatin and zeatin riboside. |
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