The relationship between HDL-, LDL-, liposomes-free cholesterol, biliary cholesterol and bile salts in the rat |
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| Authors: | C Esnault-Dupuy F Chanussot H LaFont C Chabert J Hauton |
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| Institution: | 1. Embryo Biotechnology Laboratory, Department of Physiology, College of Veterinary Science, S. V. Veterinary University, Tirupati, Andhra Pradesh, India;2. Department of Animal Genetics and breeding, College of Veterinary Science, S. V. Veterinary University, Tirupati, Andhra Pradesh, India;1. Department of Gynecological Endocrinology and Reproductive Medicine, Innsbruck Medical University, Innsbruck, Austria;2. Department of Obstetrics and Gynecology, Gadjah Mada University, Yogyakarta, Indonesia;3. Mitera IVF, Mitera Assisted Reproduction Unit, Athens, Greece;1. Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China;2. Institute of Vascular Biological Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China;3. Key Laboratory for Regenerative Medicine of the Ministry of Education, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, Hong Kong;4. Institute of Fetal-Preterm Labor Medicine, Jinan University, Guangzhou 510632, China;1. Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia;2. Center for Genomic Medicine, Copenhagen University Hospital, Copenhagen, Denmark;3. Department of Zoology and Anthropology, Constantine the Philosopher University, 949 74 Nitra, Slovakia;4. Department of Genetics and Reproduction, Research Institute of Animal Production, 949 59 Lužianky, Slovakia;5. Department of Biotechnology, University of SS. Cyril and Methodius, 917 01 Trnava, Slovakia;1. Laboratory of Animal Reproductive Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China;2. National Key Laboratory of Animal Nutrition, Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, China;3. Animal Husbandry and Veterinary Institute, Heilongjiang Academy of Land Reclamation Sciences, Harbin, China |
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| Abstract: | In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. Liposomes were used for comparison because they are assimilated by hepatocytes, but are not recognized by specific receptors. HDL isolated from rat sera were labeled with 14C]cholesterol by molecular exchange and LDL were labeled by exchange with 14C]cholesterol incorporated into phosphatidyl choline/cholesterol liposomes. The peaks of radioactivity appeared in bile 30 min after the HDL or liposome injection and after 210 min for the LDL injection. The kinetic behavior of the cholesterol carried by the liposomes was quite similar to that of cholesterol carried by HDL. Cholesterol carried by HDL was metabolized in bile salts faster than that carried by LDL: cholesterol-HDL or cholesterol-liposomes contributed to the same extent to the secretion of bile cholesterol (15 and 11%, respectively, of the injected dose), LDL (20% of the injected dose). However, the main part of 14C]cholesterol from HDL, LDL or liposomes was metabolized in bile salts. Thus, cholesterol from an exogenous source seemed to be used mainly as a substrate for bile salts. Our study revealed a difference between the hepatic metabolism of HDL, liposomes and LDL in the rat: the kinetic difference between the secretions of the radioactive compounds in bile may be explained by differences in assimilation, intracellular pathways or bile secretion. |
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