Rapid species identification of cooked poisonous mushrooms by using real-time PCR |
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| Authors: | Maeta Kazuhiko Ochi Tomoya Tokimoto Keisuke Shimomura Norihiro Maekawa Nitaro Kawaguchi Nobuhisa Nakaya Makoto Kitamoto Yutaka Aimi Tadanori |
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| Affiliation: | The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori 680-8553, Japan. |
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| Abstract: | Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h. |
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