Thioredoxin-1 attenuates indomethacin-induced gastric mucosal injury in mice |
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Authors: | Tan Aiguo Nakamura Hajime Kondo Norihiko Tanito Masaki Kwon Yong-Won Ahsan M Kaimul Matsui Hirofumi Narita Makiko Yodoi Junji |
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Institution: |
a Department of Biological Responses, Kyoto University, Institute for Virus Research, Kyoto, Japan
b Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, Kyoto, Japan
c Division of Gastroenterology, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan
d Redox Bioscience Inc., Kyoto, Japan |
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Abstract: | Indomethacin is one of non-steroidal anti-inflammatory drugs that are commonly used clinically and often cause gastric mucosal injury as a side effect. Generation of reactive oxygen species (ROS) and activation of apoptotic signaling are involved in the pathogenesis of indomethacin-induced gastric mucosal injury. Thioredoxin-1 (Trx-1) is a small redox-active protein with anti-oxidative activity and redox-regulating functions. The aim of this study was to investigate the protective effect of Trx-1 against indomethacin-induced gastric mucosal injury. Trx-1 transgenic mice displayed less gastric mucosal damage than wild type (WT) C57BL/6 mice after intraperitoneal administration of indomethacin. Administration of recombinant human Trx-1 (rhTrx-1) or transfection of the Trx-1 gene reduced indomethacin-induced cytotoxicity in rat gastric epithelial RGM-1 cells. Pretreatment with rhTrx-1 suppressed indomethacininduced ROS production and downregulation of phosphorylated Akt in RGM-1 cells. Survivin, a member of inhibitors of apoptosis proteins family, was downregulated by indomethacin, which was suppressed in Trx-1 transgenic mice or by administration of rhTrx-1 in RGM-1 cells. Trx-1 inhibits indomethacin-induced apoptotic signaling and gastric ulcer formation, suggesting that it may have a preventive and therapeutic potential against indomethacin-induced gastric injury. |
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Keywords: | Thioredoxin redox indomethacin survivin apoptosis |
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