玉米秸秆液态发酵制备麦角甾醇的研究

本文研究了利用电融合技术得到麦角甾醇工程菌株发酵玉米秸秆糖化液制备麦角甾醇的工艺条件，在摇床水平上研究了初始糖度，氮源，pH值，发酵时间对产麦角甾醇的影响。依据DPS的中心组合实验设计原理，采用4因素3水平的响应面分析方法，对工艺条件进行优化。结果显示，该4因素对麦角甾醇的积累存在显著相关性，在最佳工艺条件下摇床振荡培养32 h，生物量达8.67g/L，麦角甾醇含量可达2.37%。同时对玉米秸秆发酵产麦角甾醇晶体进行结构表征，为生物质资源的应用开辟新的途径。

Study on the Production of Ergosterol using Corn Straw Hydrolyzates Fermentation

The ergosterols were produced from corn straw hydrolysates fermented by ergosterol yeast, which was obtained from protoplast electrofusion. The effects on the yield of ergosterol were studied in the condition of shaker, such as initial sugar concentration, nitrogen source, pH value and fermentation time. The technical conditions were optimized according to the DPS center-united experimental design principles and the method of response surface analysis with four factors and three levels. The results indicated that the four factors had significant correlation to ergosterol accumulation. The biomass and the ergosterol content could be up to 8.67g/L and 2.37% respectively after cultivated for 32h under optimal technical condition. The structure of ergosterol crystal was characterized by UV, IR and SEM. A new approach of biomass source application was presented.