A lectin-binding immunoassay indicates a possible glycosylated growth hormone in the human pituitary gland |
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| Authors: | Y N Sinha U J Lewis |
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| Affiliation: | 1. Key Laboratory of Dairy Science (Northeast Agricultural University), Ministry of Education, College of Food Science, Harbin 150030, PR China;2. Lasbela University of Agriculture, water and Marine Science Uthal post code 90150, Balochistan, Pakistan;3. Northeast Agricultural University, Anal & Testing Ctr, Harbin 150030, PR China;1. Department of Integrative Oncology, BC Cancer Research Institute, Vancouver, BC, Canada;2. Interdisciplinary Oncology Program, University of British Columbia, Vancouver, BC, Canada;3. Canada''s Michael Smith Genome Sciences Centre, BC Cancer Research Institute, Vancouver, BC, Canada;4. Meyer Cancer Centre, Weill Cornell Medicine, New York City, NY, USA;5. Department of Biochemistry and Molecular Biology, Justus Liebig University Giessen, Giessen, Germany;6. Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada;7. Department of Pathology & Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada;8. Department of Biology, Stanford University, Stanford, CA, USA;9. Thoracic and GI Malignancies Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA;10. High Throughput Screening Core Facility, Memorial Sloan-Kettering Cancer Center, New York City, NY, USA;11. Keren Therapeutics, New York City, NY, USA;12. Department of Pathology, Memorial Sloan Kettering Cancer Center, New York City, NY, USA;13. Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York City, NY, USA;14. Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada;1. College of Chemical Engineering, Fuzhou University, Fuzhou 350108, China;2. College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China;1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing, 100081, People’s Republic of China;2. College of Food and Biological Engineering, Chengdu University, No.1 Shilling Road, Chenglo Avenue, Longquan District, Chengdu, 610106, People’s Republic of China |
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| Abstract: | By means of a lectin binding immunoassay we detected a glycosylated human growth hormone-immunoreactive substance in the human pituitary gland. The assay involves reacting the test substance with concanavalin A immobilized on a solid support followed by treatment with human growth hormone antibodies. Quantitation is achieved by reaction of the concanavalin A-glycoprotein-antibody complex with an 125I-labeled second antibody. Specificity of the assay for a glycosylated human growth hormone was indicated by inhibition of binding by N-acetyl-D-glucosamine. Furthermore, use of antiserum that had been absorbed with human growth hormone reduced the amount of radioactivity bound to the tubes. Western blotting of pituitary immunoprecipitates after gel electrophoresis in sodium dodecyl sulfate revealed human growth hormone-immunoreactive components that also reacted with concanavalin A. |
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