In vivo effect of the tetrapeptide, N-Acetyl-Ser-Asp-Lys-Pro, on the G1-S transition of rat hepatocytes |
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| Authors: | M-N Lombard D Sotty J Wdzieczak-Bakala† M Lenfant |
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| Institution: | Institut Curie, Centre Universitaire, F-91405 Orsay;*Institut de Chimie des Substances Naturelles, CNRS, F-91198 Gif Sur Yvette;†Institut Gustave Roussy, INSERM U2 50, rue Camille Desmoulins, F-94805 Villejuif, Cedex, France |
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| Abstract: | Abstract. The synthetic molecule N-Acetyl-Ser-Asp-Lys-Pro (Ac-SDKP), corresponding to the low molecular weight inhibitory factor preventing in vivo haematopoietic stem cell (CFU-S) entry into DNA synthesis, was tested in two heterologous systems in vivo: adult regenerating rat liver and 10-day-old rat hepatocytes synchronized by an irritating trigger. In both systems, it was shown that doses of 2–8 μg kg-1 of tetrapeptide inhibited 50–70% of the hepatocyte G1-S transitions. |
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