<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of shoot apices of Kentucky bluegrass (<Emphasis Type="Italic">Poa pratensis</Emphasis> L.) and production of transgenic plants carrying a <Emphasis Type="Italic">betA</Emphasis> gene |
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Authors: | Kewei Zhang Juan Wang Xiaorui Hu Aifang Yang Juren Zhang |
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Institution: | (1) School of Life Science, Shandong University, 27 Shanda South Road, 250100 Jinan, Shandong, China;(2) National Defense Technology Institute, Shandong University, 250100 Jinan, Shandong, China |
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Abstract: | Apical meristems of multiple shoots produced from axenic seedlings of Kentucky bluegrass (Poa pratensis L.) were used for Agrobacterium tumefaciens-mediated transformation. Transformation parameters were optimized for concentration of bacterial cells, duration of infection,
and vacuum infiltration. The highest transformation frequency (1.42%) was obtained by infection with Agrobacterium suspension of OD600 = 0.6 for 5 min, under a negative pressure of 0.5 × 105 Pa. After co-cultivation, the herbicide-resistant plants were rooted and transplanted into flowerpots. Transgenic plants
were confirmed by polymerase chain reaction (PCR) assay and Southern blot analysis. Using this transformation system, the
betA gene encoding choline dehydrogenase and mutant als gene encoding the enzyme acetolactate synthase were introduced into three Kentucky bluegrass cultivars. |
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