Influence of zinc on soluble carbohydrate and free amino acid levels in rapeseed plants regenerated in vitro in the presence of zinc |
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| Authors: | Asma Ben Ghnaya Annick Hourmant Stéphane Cerantola Nelly Kervarec Jean Yves Cabon Michel Branchard Gilbert Charles |
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| Affiliation: | 1.Université Européenne de Bretagne,Rennes,France;2.Laboratoire de Biotechnologie et Physiologie Végétales, ESMISAB, Technop?le Brest-Iroise,Université de Bretagne Occidentale,Plouzané,France;3.Laboratoire de Toxicologie Alimentaire et Cellulaire, UFR Sciences et Techniques,Université de Bretagne Occidentale,Brest Cedex 3,France;4.Laboratoire de Résonance Magnétique Nucléaire, UFR Sciences et Techniques,Université de Bretagne Occidentale,Brest Cedex 3,France;5.Laboratoire de Chimie, Electrochimie Moléculaire et Chimie Analytique—UMR 6521, UFR Sciences et Techniques,Université de Bretagne Occidentale,Brest Cedex 3,France;6.Laboratoire d’Ecophysiologie et Biotechnologie des Halophytes et Algues Marines (LEBHAM), IUEM, Technopole Brest Iroise, Place Nicolas Copernic,Université de Bretagne Occidentale,Plouzané,France |
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| Abstract: | To gain more insight into the impact of zinc on the primary metabolites in rapeseed, plants were regenerated in vitro in the presence of zinc (0.1–1 mM), acclimatized, transferred to a greenhouse, and treated with 2 mM ZnSO4. The main metabolites, including soluble carbohydrates and free amino acids, were analyzed by nuclear magnetic resonance spectroscopy, and further confirmed by spectrophotometry and enzymatic analyses. Exposure of these greenhouse-grown plants to ZnSO4 led to both a significant rise of the total amino acid level and an increase of proline accumulation in the different tissues. Moreover, the control plants, regenerated without zinc stress, exhibited a significant reduction of soluble carbohydrate levels, whereas the plants derived from Zn-treated regenerants exhibited significantly higher levels of both glucose and sucrose. These levels increased proportionally with the Zn concentration in the regeneration step. |
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