Intracellular viability of toxigenic Corynebacterium diphtheriae strains in HEp-2 cells |
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Authors: | Hirata Raphael Napoleão Fátima Monteiro-Leal Luiz Henrique Andrade Arnaldo F B Nagao Prescilla E Formiga Luiz Carlos D Fonseca Leila S Mattos-Guaraldi Ana Luíza |
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Affiliation: | Centro de Investigaciones Biológicas (CSIC), Department of Molecular Microbiology, Velázquez 144, 28006, Madrid, Spain. |
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Abstract: | We report the identification and genetic analysis of mutants in the antitoxin of the parD (kis, kid) killer system of plasmid R1. Missense mutants placed at codons 10, 11, 12 and 18 maintained the antitoxin activity of Kis, but not the ability of this protein to co-regulate the parD system together with the Kid toxin. Deletion of the last 33 amino acids of Kis inactivated the antitoxin activity of the protein and reduced substantially, but not completely, its regulatory activity. These results define two functional regions in Kis: an amino-terminal region which is specifically involved in regulation, and a carboxy-terminal region of the protein, which is important both for its regulatory and antitoxin activities. |
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Keywords: | parD operon parD regulation Kis functional region Kis, ChpAI and ChpBI relationship Toxin–antitoxin system |
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