Peptide nucleic acids (PNAs) antisense effect to bacterial growth and their application potentiality in biotechnology |
| |
Authors: | Masashi Hatamoto Akiyoshi Ohashi Hiroyuki Imachi |
| |
Institution: | (1) Department of Social and Environmental Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima, Hiroshima 739-8527, Japan;(2) Subsurface Geobiology Advanced Research (SUGAR) Team, Extremobiosphere Research Program, Institute of Biogeosciences, Japan Agency for Marine–Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan |
| |
Abstract: | Peptide nucleic acids (PNAs) are nucleic acid analogs having attractive properties such as quiet stability against nucleases
and proteases, and they form strong complexes with complementary strands of DNA or RNA. Because of this attractive nature,
PNA is often used in antisense technology to inhibit gene expression and microbial cell growth with high specificity. Many
bacterial antisense or antiribosomal studies using PNA oligomers have been reported so far, and parameters to design effective
antisense PNAs and to improve PNA cell entry for efficient inhibition of bacterial growth have been presented. However, there
are still several obstacles such as low cellular uptake of PNA while applying antisense PNAs to a complex microbial community.
On overcoming these problems, the PNA antisense technique might become a very attractive tool not only for controlling the
microbial growth but also for further elucidating microbial ecology in complex microbial consortia. Here, we summarize and
present recent studies on the development of antimicrobial PNAs targeting mRNAs and rRNAs. In addition, the application potentiality
of antisense techniques in nonclinical biotechnology fields is discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|