Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry |
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Authors: | Daniela Lopes Paim Pinto Beatriz de Almeida Barros Lyderson Facio Viccini José Marcello Salabert de Campos Maurecilne Lemes da Silva Wagner Campos Otoni |
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Affiliation: | 1.Laboratório de Cultura de Tecidos Vegetais,Departamento de Biologia Vegetal/BIOAGRO,Vi?osa,Brazil;2.Laboratório de Biologia Molecular e Filogeografia/BIOAGRO,Vi?osa,Brazil;3.Laboratório de Genética, Instituto de Ciências Biológicas,Departamento de Biologia, Universidade Federal de Juiz de Fora,Juiz de Fora,Brazil;4.Laboratório de Genética, Departamento de Ciências Biológicas,Universidade do Estado do Mato Grosso,Tangará da Serra,Brazil |
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Abstract: | In this study, flow cytometric analysis was used to evaluate the genetic stability of Passiflora cincinnata Mast. plants regenerated via primary and secondary somatic embryogenesis. Embryogenic calli obtained from culturing zygotic embryos on Murashige and Skoog (MS) medium containing 18.1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.4 μM benzyladenine (BA) were transferred to differentiation medium. Torpedo and cotyledonary embryos were obtained. These primary embryos were maintained on differentiation medium to generate secondary embryos. Conversion of primary and secondary embryos yielded 305 and 138 normal plants, respectively. Almost 90% of plantlets survived following acclimatization. Flow cytometric analysis revealed that seed-derived plants had on average 3.01 pg nuclear DNA (2C), and all plants, except for a single plant regenerated via primary embryogenesis, maintained their ploidy. This single plant contained more than twice the average DNA content: 6.21 pg (4C). Epidermal stomata of leaves of the tetraploid plant were larger but lower in density than those of diploid plants, indicating that stomatal characteristics are useful in distinguishing between diploid and tetraploid plants of passion fruit. In summary, the procedure we employed to regenerated P. cincinnata plants via somatic embryogenesis generated mostly genetically true-to-type plants. |
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