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Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection |
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| Authors: | Natacha Olieric Melanie Kuchen Sandro Wagen Marion Sauter Stephanie Crone Sonia Edmondson Daniel Frey Christian Ostermeier Michel O Steinmetz Rolf Jaussi |
| Affiliation: | 1.Paul Scherrer Institut, Biomolecular Research,Villigen PSI,Switzerland;2.Institute of Biotechnology, Zurich University of Applied Sciences,W?denswil,Switzerland;3.Novartis Institutes for Biomedical Research,Basel,Switzerland |
| Abstract: | BackgroundMolecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. We have established an automated, fast and flexible low-cost expression cloning approach requiring only vector and insert amplification by PCR and co-transformation of the products. |
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