| 霍乱肠毒素B亚单位在转基因番茄中表达的研究 |
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| 引用本文： | 彭志强,贺竹梅,俞守义,余迪求,李宝健.霍乱肠毒素B亚单位在转基因番茄中表达的研究[J].生命科学研究,2001,5(3):259-264. |
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| 作者姓名： | 彭志强 贺竹梅 俞守义 余迪求 李宝健 |
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| 作者单位： | 1. 第一军医大学热带军队卫生学系,
2. 中山大学生物工程研究中心, |
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| 基金项目： | 广东省科学研究基金资助项目(001212) |
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| 摘 要： | 将霍乱肠毒素B亚单位（CT－B）基因及内质网引导序列（SEKDEL）克隆到质粒pRTL2和pBI121中，分别构建植物双元表达载体pBI-CTB和pBI-CTBK,CT-B基因由Ca35S启动子控制表达。采用叶盘法经根癌农杆菌介导转化番茄（金丰1号，Jinfeng1)各表达载体得到一批转基因植株。经PCR和Southern blot分析表明CT－B基因整合到了番茄基因组中；ELISA和Western blot分析表明pBI-CTB和pBI-CTBK的转基因植株能够有效表达CT－B多肽，分别占番茄叶片可溶性蛋白的0.055%和0.084%。
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| 关 键 词： | 霍乱肠毒素B亚单位 转基因番茄 基因表达 |
| 文章编号： | 1007-7847(2001)03-0259-06 |
| 修稿时间： | 2001年4月20日 |
Expression of Cholera Toxin B Subunit in Transgenic Tomato Plants |
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| PENG Zhi qiang ,HE Zhu mei ,YU Shou yi ,YU Di qiou ,LI Bao jian.Expression of Cholera Toxin B Subunit in Transgenic Tomato Plants[J].Life Science Research,2001,5(3):259-264. |
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| Authors: | PENG Zhi qiang HE Zhu mei YU Shou yi YU Di qiou LI Bao jian |
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| Institution: | PENG Zhi qiang 1,HE Zhu mei 2,YU Shou yi 1,YU Di qiou 2,LI Bao jian 2 |
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| Abstract: | The cholera toxin B subunit(CT B) gene was subcloned into middle vector pRTL2 and binary vector pBI121 to form pBI CTB and pBI CTBK. Two plant transformation vector (pBI CTB, pBI CTBK) were constructed to express CT B under the control of the CaMV 35S promoter. The tomato plant (Jinfeng1) cotyledon were transformed by co cultivating leaf discs method with Agrobacterium strains harboring pBI CTB and, pBI CTBK respectively. The regenerated kanamycin resistant tomato transformants were analyzed by PCR, Southern blot, ELISA and Western blot.These results indicated the CT B gene integrated in the tomato genomic DNA and was expressed. CT B lever in the pBI CTB and pBI CTBK lines were up to 0.055% and 0.084% of the total soluble tomato leaf protein. |
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| Keywords: | cholera toxin B subunit transgenic tomato gene expression |
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