Proteolytic activity of rumen fungi belonging to the genera Neocallimastix and Piromyces |
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| Authors: | N Asao K Ushida Y Kojima |
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| Institution: | Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606, Japan |
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| Abstract: | Proteolytic activity of two rumen fungal isolates Neocallimastix sp. strain N1 and Piromyces sp. strain P1 was examined. Proteases are active between pH 6.5 and 9.0 with maximum at 7.9 for isolate N1 and between 6.5 and 10.5 with maximum at 8.8 for isolate P1. Proteolytic activity increased as temperature increased until 50°C and a sudden decrease at 60°C was observed in both isolates. EDTA, 1,10-phenanthroline, p -chloromercuribenzoate (PCMB), merthiolate and phenylmethylsulphonyl fluoride (PMSF) were effective proteolytic inhibitors against both isolates. |
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