AFLP assessment of genetic variability among velvetbean (Mucuna sp.) accessions |
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Authors: | L J A Capo-chichi D B Weaver C M Morton |
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Institution: | (1) 202 Funchess Hall, Department of Agronomy and Soils, Auburn University, Auburn University, AL. 36849-5412, USA e-mail: cludovic@acesag.auburn.edu Tel: +1-334 844-3982, Fax: +1-334 844-3945, US;(2) 101 Life Sciences Building, Department of Biological Sciences, Auburn University, Auburn University, AL. 36849-5412, USA, US |
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Abstract: | Velvetbean (Mucuna sp.) is a self-pollinated crop classified within the Leguminosae. Using AFLP markers, gene diversity and phenetic relationships
were estimated in a collection of 40 velvetbean accessions from cultivated species and different eco-geographic regions. Eleven
selective primer combinations generated a total of 508 amplification products. The average number of scorable fragments was
23 per primer combination. A total of 251 polymorphic markers was detected. The polymorphisms obtained ranged from 36% to
61% with an average of 49%. The final phenetic trees were constructed using Nei and Li’s coefficient of similarity with UPGMA.
Other clustering algorithms were examined and all had high co-phenetic correlations, indicating the goodness of fit for the
resulting phylogenetic trees. The phenetic tree as well as principal component analysis (PCA) separated the 40 velvetbean
accessions into two main clusters. Bootstrap and Jackknife analyses were completed and their values indicated strong to moderate
support for the two main clusters. This grouping confirmed the existing phenological difference with regard to maturity. The
high values of the similarity coefficients observed (0.87 to 0.97) imply that the accessions used in this study are similar.
The level of genetic variability detected within the velvetbean accessions with AFLP analysis suggests that it is a reliable,
efficient, and effective marker technology for determining genetic relationships in velvetbean.
Received: 19 June 2000 / Accepted: 1 March 2001 |
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Keywords: | Velvetbean Mucuna sp AFLP Genetic diversity Polymorphism Phenetic |
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