Distribution of glycosyltransferase activities in different compartments of mitochondria |
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Authors: | C Levrat P Louisot R Morelis |
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Institution: | Department of Biochemistry, University of Lyon, INSERM-CNRS U 189. Lyon-Sud Medical School, Oullins, France. |
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Abstract: | The liver mitochondria were submitted to a first swelling which allowed to get outer membranes. The mitoplasts obtained in these conditions were subject to a second swelling. The separation of submitochondrial membranes on a discontinuous sucrose gradient revealed three membrane fractions, an outer membrane rich fraction, an inner membrane rich fraction and a fraction enriched with contact sites between the two membranes. The various glycosyltransferase systems involved in the biosynthesis of N-glycoproteins were located in these fractions. |
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