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拟南芥2-甲基-6-叶绿基-1,4-苯醌甲基转移酶启动子的分离及表达特性分析
引用本文:刘 宾,王 磊,杨娇艳,张 伟,范云六. 拟南芥2-甲基-6-叶绿基-1,4-苯醌甲基转移酶启动子的分离及表达特性分析[J]. 生物工程学报, 2008, 24(1): 33-39. DOI: 10.1016/S1872-2075
作者姓名:刘 宾  王 磊  杨娇艳  张 伟  范云六
作者单位:1. 中国农业科学院生物技术研究所,北京,100081;河北大学生命科学学院,保定,071002
2. 中国农业科学院生物技术研究所,北京,100081;中国农业科学院生物技术研究所,北京,100081
3. 中国农业科学院生物技术研究所,北京,100081;华中师范大学,武汉,430000
基金项目:国家重点基础研究发展规划(973项目)资助项目(No. 2004CB117203).
摘    要:维生素E是一类人体必需的脂溶性抗氧化剂, 具有重要的生理功能。2-甲基-6-叶绿基-1,4-苯醌甲基转移酶(MPBQ MT)是天然维生素E合成途径中的关键酶之一, 催化MPBQ甲基化, 生成DMPBQ。从拟南芥分离了MPBQ MT基因1018bp的启动子序列, 构建了含该启动子和GUS报告基因的植物表达载体, 通过农杆菌介导转化拟南芥, 获得了转基因植株。GUS组织化学染色结果表明, 在MPBQ MT启动子驱动下, 报告基因GUS在拟南芥的茎、叶、花萼、雄蕊、种荚均有表达, 且在茎、叶、种荚中表达量较高, 而在根、花瓣和种子中则没有观察到GUS基因的表达, 表明MPBQ MT基因可能仅在拟南芥幼嫩茎、叶、种荚等绿色组织中特异性高表达。

关 键 词:拟南芥   维生素E   生育酚   2-甲基-6-叶绿基-1  4-苯醌甲基转移酶(MPBQ MT)   启动子
收稿时间:2007-04-20
修稿时间:2007-06-04

Isolation and Characterization of 2-methyl-6-phytyl-1, 4-benzoquinol Methyltransferase Gene Promoter from Arabidopsis thaliana
Bin Liu,Lei Wang,Jiaoyan Yang,Wei Zhang and Yunliu Fan. Isolation and Characterization of 2-methyl-6-phytyl-1, 4-benzoquinol Methyltransferase Gene Promoter from Arabidopsis thaliana[J]. Chinese journal of biotechnology, 2008, 24(1): 33-39. DOI: 10.1016/S1872-2075
Authors:Bin Liu  Lei Wang  Jiaoyan Yang  Wei Zhang  Yunliu Fan
Affiliation:Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China; 3 College of Life Sciences, Hebei University, Baoding 071002, China;Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China; 4 Huazhong Normal University, Wuhan 430000, China;Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China
Abstract:Vitamin E, or tocopherol, is a lipid-soluble antioxidant and essential for human health. 2-methyl-6-phytyl-1, 4-benzo- quinol methyltransferase (MPBQ MT), one of the key enzymes in vitamin biosynthetic pathway in plants, converts 2-methyl- 6-phytyl-1,4-benzoquinol(MPBQ) to 2,3-dimethyl-6-phytyl-1,4-benzoquinol (DMPBQ).In this study, we isolated the 1018bp promoter of Arabidopsis MPBQ MT gene. The promoter was fused with GUS reporter gene and this expression cassette was introduced into wild Arabidopsis by Agrobacterium-mediated transformation. GUS staining shows that GUS gene was expressed in stems, leaves, calyxes, stamens and siliques in transgenic Arabidopsis plants, with higher expression in green tissue such as stems, leaves, siliques, while invisible in roots, petals, and seeds. The data indicate that MPBQ MT gene promoter was likely to be expressed preferentially in green tissues such as stems, leaves, and young siliques.
Keywords:Arabidopsis thaliana   Vitamin E   Tocopherol   2-methyl-6-phytyl-1  4-benzoquinol methyltransferase (MPBQ MT)   Promoter
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