Effects of antioxidants and reduced oxygen tension on rat mammary epithelial cells in culture |
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Authors: | Tzu-Ping Lin Yun Kit Hom James Richards Satyabrata Nandi |
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Institution: | (1) Department of Molecular and Cellular Biology and Cancer Research Laboratory, University of California at Berkeley, 447 Life Sciences Addition, 94720 Berkeley, California |
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Abstract: | Summary Free radical damage has the potential to significantly affect the behavior of cells in culture. In this study the effects
of antioxidants (superoxide dismutase, catalase, and vitamin E) and lowered oxygen tension (1% oxygen) on primary culture
of rat mammary epithelial cells were examined. Rat mammary epithelial cells were dissociated in collagenase with or without
the addition of antioxidants and low oxygen tension, then cultured for 10 d in rat-tail collagen gel matrix and fed with Dulbecco’s
modified Eagle’sF12 medium supplemented with various hormones and growth factors. Growth potential of the mammary cells was
enhanced when antioxidants and low oxygen tension were used, alone or in combination, during the cell dissociation period.
Using antioxidants and low oxygen tension during the culture period failed to improve growth potential regardless whether
cells were dissociated in standard conditions or with antioxidants and low oxygen tension. The use of antioxidants and low
oxygen tension during the cell dissociation period also reduced the degree of keratinization of the cells after 10 d of culture.
Using antioxidants and low oxygen tension during the cell culture period did not further reduce keratinization if antioxidants
and low oxygen tension were used during the dissociation period, but were effective in reducing keratinization if cells were
dissociated in standard condition. In this system, antioxidants and low oxygen tension reduced lipid peroxidation during the
cell dissociation period. An iron chelator, desferal, can also reduce lipid peroxidation and enhance growth when used during
cell dissociation, suggesting the enhanced growth potential by the addition of antioxidants and low oxygen to be due to the
reduction of lipid peroxidation.
This study is supported by grants CA05388 and GM11903 to Y. K. H. from the Public Health Service, U.S. Department of Health
and Human Services, Washington, DC. |
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Keywords: | antioxidants rat mammary primary culture |
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