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Identification of protein S 1 at the messenger RNA binding site of the Escherichia coli ribosome

Authors:	I Fiser KH Scheit G Stöffler E Kuechler

Institution:	1. Institut für Biochemie der Universität Wien, Währinger Straße 17, A-1090 Wien, Austria;2. Max-Planck-Institut für Biophysikalische Chemie, Göttingen, GermanyGFR;3. Max-Planck-Institut für Molekulare Genetik, Berlin, Germany

Abstract:	Poly-4-thiouridylic acid acts as messenger RNA for polyphenylalanine synthesis in an $in vitro$ protein synthesizing system. When a complex consisting of ribosomes, poly-4-thiouridylic acid and Phe-tRNA is irradiated at 300 to 400 nm, covalent bonds between this messenger RNA and protein S 1 are formed.

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