Comparative evaluation of gene delivery devices in primary cultures of rat hepatic stellate cells and rat myofibroblasts |
| |
Authors: | Ralf Weiskirchen Jens Kneifel Sabine Weiskirchen Eddy van de Leur Dagmar Kunz Axel M Gressner |
| |
Affiliation: | (1) Molecular Mechanisms of Disease Laboratories, Department of Pathology, Stanford University Medical School, 300 Pasteur Drive, Stanford, CA 94305-5324, USA |
| |
Abstract: | Background The cell surface undergoes continuous change during cell movement. This is characterized by transient protrusion and partial or complete retraction of microspikes, filopodia, and lamellipodia. This requires a dynamic actin cytoskeleton, moesin, components of Rho-mediated signal pathways, rearrangement of membrane constituents and the formation of focal adhesion sites. While the immunofluorescence distribution of endogenous moesin is that of a membrane-bound molecule with marked enhancement in some but not all microextensions, the C-terminal fragment of moesin co-distributes with filamentous actin consistent with its actin-binding activity. By taking advantage of this property we studied the spontaneous protrusive activity of live NIH3T3 cells, expressing a fusion of GFP and the C-terminal domain of moesin. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|