Isolation and characterization of 11 polymorphic microsatellite loci from Fatsia polycarpa (Araliaceae), an element of evergreen forests in Taiwan |
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Authors: | Chi-Chun Huang Kuo-Hsiang Hung Tsai-Wen Hsu Kuo-Hsiung Wang Chi-Yung Lin Tzen-Yuh Chiang |
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Affiliation: | (1) Department of Life Sciences, Cheng Kung University, Tainan, 701, Taiwan;(2) Taiwan Endemic Species Research Institute, Nantou, 552, Taiwan |
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Abstract: | Fatsia polycarpa Hayata (Araliaceae) is an element of evergreen forests at middle elevations in Taiwan, also representing the southern limitation in distribution of the genus. In this study, we described the development of 11 microsatellite loci from F. polycarpa in Taiwan for genetic studies. These new markers were tested in 20 individuals of 5 populations of the woody species. The number of alleles ranged from 9 to 18. The expected (H E ) and observed (H O ) heterozygosities were 0.80–0.95 and 0.10–0.75, respectively. Six of the 11 microsatellite loci are significantly deviated from Hardy–Weinberg expectations likely due to the population structure within samples. No linkage disequilibrium were observed from pairwise comparisons of loci. Chi-Chun Huang, Kuo-Hsiang Hung, and Tsai-Wen Hsu contributed equally to this article. |
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Keywords: | Fatsia polycarpa Microsatellite PIMA RAPD-PCR enrichment Taiwan |
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