昆虫病原线虫斯氏线虫和异小杆线虫对长角血蜱雌蜱的致病力

用昆虫病原线虫小卷蛾斯氏线虫（Sc BJ）、夜蛾斯氏线虫（Sf Otio）、拟双角斯氏线虫（Sc D43）、格氏斯氏线虫（Sg NC32）和嗜菌异小杆线虫（Hb E-6-7）对长角血蜱雌蜱进行感染试验，所用线虫剂量为4 000 Ijs/dish。结果表明，5种线虫均对长角血蜱雌蜱有致死效应。Hb E-6-7和Sc BJ两种线虫对雌蜱各发育期致病力最强，导致雌蜱的累积死亡率和半致死时间分别为饥饿雌蜱82.5%，9.0天和75.0%，8.8天；吸血雌蜱90.0%，8.0天和82.5%，8.0天；饱血雌蜱93.3%，7.3天和86.7%，7.3天。Sc D43对饱血雌蜱有较高的致死效应，为80.0%，但半致死时间较长，为11.7天。Sf Otio和Sg NC32对长角血蜱雌蜱的致死效应较低。饱血雌蜱较饥饿雌蜱和吸血雌蜱更易被线虫感染。

Virulence of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) to female ticks of Haemaphysalis longicornis Neumann (Acari:Ixodidae)

Infection of entomopathogenic nematodes Steinernema carpocapsae BJ(Sc BJ) ,S. feltiae Otio(Sf Otio),S. ceratophorum D43(Sc D43) and S. gla seri NC32(Sg NC32) (Steinernematidae),and Heterorhabditis bacteriophora E- 6-7 (Hb E-6-7) (Heterorhabditidae) to female Haemaphysalis longicornis Neumann was conducted. The nematodes dosage used was 4 000 IJs/dish. The results showed that the five entomopathogenic nematodes had lethal effects on female H .longicornis. Hb E-6-7 and Sc BJ were the most virulence species to various developmental stages of female ticks: the cumulative mortality and the LT_(50 ) of u nfed female ticks from nematodes infection were 82.5%,9.0 d and 75.0%,8.8 d, respectively; feeding female 90.0%,8.0 d and 82.5%,8.0 d; engorged fema le 93.3%,7.3 d and 86.7%,7.3 d. Sc D43 infection also caused high mortalit y (80.0%) to engorged female ticks, but the LT_(50) was relatively long (11 . 7 d). Lethal effects of Sf Otio and Sg NC32 to this tick were low. Engorged fem ale ticks were more sensitive than unfed and feeding female ticks.