霍乱弧菌分型噬菌体VP3蛋白的双向电泳分析

目的:噬菌体-生物分型方案具有区分霍乱弧菌潜在致病力的作用,VP3是5个霍乱弧菌分型噬菌体之一。对VP3成熟颗粒的蛋白组成进行测定和分析,以补充基因组注释信息。方法:在全基因组测序及生物信息学分析的基础上,利用双向电泳技术及质谱鉴定,对纯化的成熟VP3噬菌体的结构蛋白进行分离及鉴定。结果:双向电泳分离得到近20个蛋白点,质谱鉴定出了其中的10个,对应于4个VP3蛋白和4个霍乱弧菌蛋白。结论:VP3结构蛋白的组成和T7具有很高的相似性。与噬菌体颗粒一起被纯化分离的宿主蛋白可能在VP3的转染过程中起作用。

The Two-Dimension Electrophoresis Analysis of VP3, the Typing Phage of Vibrio cholerae

Objective:The phage-biotyping scheme can differentiate the potential pathogenicity of different Vibrio cholerae strains.VP3 is one of the five typing phages.To complement the annotation of the VP3 genome,the protein composition was analyzed.Methods:On the basis of complete genome sequencing and bioinformation analysis,the structure proteins of the purified mature VP3 particles were separated and identified using two-dimension electrophoresis and MS.Results:20 protein spots were separated and 10 of them were identified by MALDI-MS/MS,which corresponded to four proteins of VP3 and four proteins of V.cholerae.Conclusion:The results indicate that there was high similarity between the structure protein compositions of VP3 and T7.The host proteins purified with the phage particles may play some roles in the transduction of VP3.