Cytoplasmic control of nuclear maturation in mouse oocytes |
| |
Authors: | Hanna B
akier Renata Czo
owska |
| |
Institution: | Hanna B
akier,Renata Czo
owska |
| |
Abstract: | Oocytes with germinal vesicles were cut into anucleate and nucleate fragments. At the time of germinal vesicle breakdown (GVBD) in nucleate fragments (after 2–3 h of culture) sister anucleate fragments were fused with the help of inactivated Sendai virus with interphase blastomeres from 2-cell embryos. The hybrid cells were examined after
h and 20 h. The anucleate fragments induced chromosome condensation in the nuclei of interphase blastomeres immediately after fusion. On this basis it may be concluded that GVBD and nuclear maturation in mouse oocytes is induced by a cytoplasmic factor which is produced or unmasked independently of the nucleus. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |