Various cationic carriers for in vitro transfection of tumor and endothelial cell lines |
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| Authors: | Zemlińska Barbara Sochanik Aleksander Missol-Kolka Ewa Szala Stanisław |
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| Affiliation: | Department of Molecular Biology, Center of Oncology-Maria Sk?odowska-Curie Memorial Institute, Gliwice, Poland. |
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| Abstract: | We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE. |
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