Gas-liquid chromatographic analysis of cyasin in cycad flour |
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Authors: | W W Wells M G Yang W Bolzer O Mickelsen |
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Institution: | 1. Department of Biochemistry, Michigan State University, East Lansing, Michigan 48823 USA;2. Department of Foods and Nutrition, Michigan State University, East Lansing, Michigan 48823 USA |
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Abstract: | A method for the quantitative determination of cycasin from cycad flour by gas-liquid chromatography is described. The flour is extracted with 70% ethanol and the residue from the dried extract is directly trimethylsilylated. Androsterone was found to be an excellent internal standard. The average content of cycasin from ten separate analyses of one lot of flour was . The method is rapid, sensitive, and not hindered by contaminating compounds. |
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