Triton-stimulated nucleoside diphosphatase activity: Subcellular localization in corn root homogenates |
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| Authors: | J. Nagahashi A. P. Kane |
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| Affiliation: | (1) Eastern Regional Research Center, A.R.S., U.S.D.A., 600 East Mermaid Lane, 19118 Philadelphia, PA, USA;(2) Department of Biology, Williams College, Williamstown, Massachusetts |
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| Abstract: | Summary The distribution of latent UDPase activity (cold storage-activated) is similar to Triton-stimulated UDPase activity in membrane fractions separated by differential centrifugation as well as fractions purified by linear sucrose density centrifugation. The Triton-stimulated UDPase activity appears to be a specific marker for Golgi membranes in corn root homogenates. Detergent-activated UDPase activity provides a more reliable, less cumbersome way to monitor Golgi membranes compared to cold storage-activation and this marker can be used on fresh preparations.This research was supported in part by NSF grant CDP-7927121 and funds received from the Bronfman Science Center, Williams College. |
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| Keywords: | Golgi Latency Membranes Nucleoside Diphosphatase Triton X-100 |
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