DNA methylation increases throughout Arabidopsis development |
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Authors: | L?Ruiz-García M?T?Cervera Email author" target="_blank">J?M?Martínez-ZapaterEmail author |
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Institution: | (1) Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, CSIC. Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain;(2) Present address: Genética Forestal. Centro de Investigaciones Forestales, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Carretera de La Coruña Km 7.5, 28040 Madrid, Spain |
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Abstract: | We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. |
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Keywords: | Arabidopsis 5-azacytidine ddm1 mutant DNA methylation pattern Plant development |
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