Biodegradation of 2,4-dichlorophenol by a <Emphasis Type="Italic">Bacillus</Emphasis> consortium |
| |
Authors: | Yesenia Herrera Anthony I Okoh Laura Alvarez Norma Robledo María R Trejo-Hernández |
| |
Institution: | (1) Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca, Morelos, 62209, Mexico;(2) Centro de Investigación en Ciencias Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca, Morelos, 62209, Mexico;(3) Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice, 5700, South Africa;(4) Centro de Recursos Bióticos, Instituto Politécnico Nacional, C.P. 62731, Yautepec, Morelos, Mexico |
| |
Abstract: | As part of our effort at establishing microbial consortia of relevance for the bioremediation of xenobiotics polluted environments
in Mexico, we assessed the aerobic biodegradation of 2,4-dichlorophenol (2,4-DCP) by a consortium of four Bacillus species
that were isolated from a polluted soil by enrichment using a mixture of chlorophenols. The bacterial consortium effectively
biodegraded 2-chlorophenol, 3-chlorophenol and 2,4-dichlorophenol at degradation rates of between 1.7 and 6.7 μmoles l−1 h−1. In the presence of NH4Cl or KNO2 as nitrogen sources, 2,4-DCP was variously degraded. Under both conditions, cell biomass attained highest values of 350 and 450 mg l−1 respectively, while the amounts of 2,4-DCP metabolized in 21 days reached peak values of 2.1 and 2.5 mM representing between
70 and 85% degradation respectively. Chloride releases during the same period were highest at 4.7 mM and 5.3 mM in the presence
of the two nitrogen sources. The presence of free-chloride in the culture medium had a significant impact on the catabolism
of 2,4-dichlorophenol. |
| |
Keywords: | 2 4-Dichlorophenol Bacillus consortium Nitrogen source Biodegradation |
本文献已被 SpringerLink 等数据库收录! |
|