Cold sensitive mutant of Escherichia coli with altered RNA polymerase |
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Authors: | V. G. Nikiforov E. S. Kalyaeva V. V. Velkov |
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Affiliation: | (1) Kurchatov Institute of Atomic Energy, Moscow, USSR |
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Abstract: | Summary Cold sensitive (cs) mutants of E. coli unable to grow at 26° C were isolated. Three of about 200 mutants tested have RNA polymerase which is about 5 times less active at low temperatures than the wild type RNA polymerase. One of such mutants cs 1 was studied in more detail. The mutation affects core enzyme and is closely linked to rif-r mutations. By transduction analysis the following order of markers was established: argH — rif-r — cs 1. |
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