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Preparation of conditioned medium to stimulate anthocyanin production using suspension cultures of Fragaria ananassa cells |
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| Authors: | Mori Tsukasa Sakura Miei |
| Affiliation: | (1) Laboratory of Physiology and Ecology, Faculty of Fisheries, Hokkaido University, Hakodate, 041, Japan;(2) Environmental Process Development Department, Industrial Machine & Plant Development Center, Ishikawajima Harima Heavy Industries, Co. Ltd., Yokohama, 235, Japan |
| Abstract: | A conditioned medium (CM) prepared from strawberry suspension cultures greatly stimulated anthocyanin accumulation. CM separated by dialysis membrane showed a significant increase (p 0.05) in anthocyanin synthesis at a fraction smaller than 10,000 Da. The stimulation by CM was eliminated when the CM was treated with alkali. |
| Keywords: | Anthocyanin cell culture conditioned medium strawberry |
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