首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Immunohistochemical study on a macrophage calcium-type lectin in mouse embryos: transient expression in chondroblasts during endochondral ossification
Authors:Shigeki Mizuochi  Yoshihiro Akimoto  Yasuyuki Imai  Hiroshi Hirano  Tatsuro Irimura
Institution:(1) Department of Cancer Biology and Molecular Immunology (formerly Division of Chemical Toxicology and Immunochemistry), Faculty of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku Tokyo, 113, Japan;(2) Department of Anatomy, Kyorin University School of Medicine, Mitaka Tokyo, 181, Japan
Abstract:We investigated expression of mouse macrophage galactose/N-acetylgalactosamine-specific calcium-type lectin (MMGL) in mouse embryos using a rat monoclonal antibody (mAb) LOM-14 that we previously developed. Immunoblot analysis revealed that a significant expression of MMGL was first detected in detergent extracts of whole embryos of 11 days post coitus (dpc) and the level of its expression increased during further fetal development (examined up to 18-dpc embryos). Tissue sections of 12, 14, 16, and 18-dpc embryos, newborn and adult mice were investigated by immunohistochemical staining. In embryos of 12-dpc and later stages, mesenchymal cells (typically distributed in the embryonic skin) exhibited positive signals for MMGL. Interestingly, a conspicuous staining was observed during endochondral ossification in temporary cartilage tissue, in which chondroblasts were transiently positive for MMGL. The staining intensity for the chondroblasts peaked in 14-dpc embryos and then gradually decreased. The staining was diminished while hypertrophy and maturation of chondrocytes proceeded, and was eliminated in areas with calcification. Immunoelectron microscopic study demonstrated the presence of MMGL in rough endoplasmic reticulum in the chondroblasts in the temporary cartilage tissue in 14-dpc embryos. These results provide first evidence showing the expression of MMGL in cells other than macrophages. © 1998 Rapid Science Ltd
Keywords:calcium-type lectin  macrophage marker  embryo  chondroblasts  endochondral ossification  immunohistochemistry  mouse
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号