The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant. |
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| Authors: | Z L Wu T C Charles H Wang E W Nester |
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| Affiliation: | Department of Microbiology, University of Washington, Seattle 98195. |
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| Abstract: | A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis. |
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