Abstract: | The responses of leaf water parameters to drought were examined using three sunflower (Helianthus annuus L.) genotypes. Osmotic potential at full water saturation (π100), apoplastic water fraction (AWF) and bulk elastic modulus (BEM) were determined by pressure-volume curve analysis on well
watered or on water-stressed plants (−1.0 MPa Ψ1 < −1.5 MPa) previously drought-pretreated or not. The drought-pretreated plants were subjected to a 7-day drought period
(predawn leaf water potential reached −0.9 MPa) followed by 8 days of rewatering. In well watered plants, all genotypes in
response to drought acclimation displayed a significantly decreased π100 associated with a decrease in the leaf water potential at the turgor-loss point (decrease in Ψtlp was between 0.15 and 0.21 MPa, depending on the genotype). In two genotypes, drought acclimation affected the partitioning
of water between the apoplastic and symplastic fractions without any effect on the total amount of water in the leaves. As
a third genotype displayed no modification of AWF and BEM after drought acclimation, the decreased π100 was only due to the net accumulation of solutes and was consistent with the adjustment of the photochemical efficiency observed
previously in this genotype in response to drought acclimation. In water-stressed plants, the osmotic adjustment (OA) can
increase further beyond that observed in response to the drought pretreatment. However, the maintenance of photosynthetic
rate and stomatal conductance at low leaf water potentials not only depends on the extent of osmotic adjustment, but also
on the interaction between OA and AWF or BEM. Adaptative responses of leaf water parameters to drought are thus quite contrasted
in sunflower genotypes.
This revised version was published online in June 2006 with corrections to the Cover Date. |