Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101 |
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| Authors: | C.-K. Chen H. P. Blaschek |
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| Affiliation: | (1) Department of Food Science and Human Nutrition, University of Illinois, 1207 W. Gregory Drive, 488 ASL, MC-630, Urbana, IL 61801, USA e-mail: blaschek@uiuc.edu Tel.:+1-217-333-8224 Fax: +1-217-244-2517, US |
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| Abstract: | Addition of sodium acetate to chemically defined MP2 medium was found to increase and stabilize solvent production by Clostridium beijerinckii BA101, a solvent-hyperproducing mutant derived from C. beijerinckii NCIMB 8052. C. beijerinckii BA101 demonstrated a greater increase in solvent production than C. beijerinckii NCIMB 8052 when sodium acetate was added to MP2 medium. In 1-l batch fermentations, C. beijerinckii BA101 produced 32.6 g/l total solvents, with butanol at 20.9 g/l, when grown in MP2 medium containing 60 mM sodium acetate and 8% glucose. To our knowledge, these values represent the highest solvent and butanol concentrations produced by a solventogenic Clostridium strain when grown in batch culture. Received: 29 September 1998 / Received revision: 13 February 1999 / Accepted: 26 February 1999 |
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