Development of Diversity Arrays Technology markers as a tool for rapid genomic assessment in Nile tilapia,Oreochromis niloticus |
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| Authors: | C. E. Lind A. Kilian J. A. H. Benzie |
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| Affiliation: | 1. WorldFish, Bayan Lepas, Penang, Malaysia;2. Diversity Arrays Technology Pty Ltd (DArT P/L), University of Canberra, Bruce, ACT, Australia;3. School of Biological Earth and Environmental Sciences, University College Cork, Cork, Ireland |
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| Abstract: | The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype‐by‐sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain‐specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs. |
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| Keywords: | aquaculture DArTseq genetically improved farmed tilapia genotype‐by‐sequencing
SNP
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