Question 3: The Worlds of the Prebiotic and Never Born Proteins |
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| Authors: | Cristiano Chiarabelli Davide De Lucrezia |
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| Institution: | (1) Centro Studi e Ricerche “E. Fermi”, Via Panisperna 89, 00184 Rome, Italy;(2) Department of Biology, V.le G. Marconi 446, University of Roma TRE, 00146 Rome, Italy |
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| Abstract: | Starting from the statement that no reliable methods are known to produce high molecular weight polypeptides under prebiotic
conditions, a possible approach, at least to understand the differences between extant proteins and the possible large number
of never born proteins, could be biological. Using the phage display method a large library of totally random amino acidic
sequences was obtained. Consequently, different experiments to directly consider the frequency of stable folds were performed,
and the interesting results obtained from such new approach are discussed in terms of contingency, contributing to the discussion
on the selection mechanism of extant proteins.
Presented at the International School of Complexity – 4th Course: Basic Questions on the Origins of Life; “Ettore Majorana”
Foundation and Centre for Scientific Culture, Erice, Italy, 1–6 October 2006. |
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| Keywords: | Macromolecular sequences Never born proteins Phage display Prebiotic synthesis Random polypeptides fold Thrombin selection |
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